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In a second simulation we investigate the performance of the IVW method and MR-Egger regression under the causal null β = 0 in scenario (c), with a fixed sample size of N = 2000 but varying the number of genetic variants. The results are shown in Table 2. The bias of the IVW estimator reduces by just under 20% as the number of genetic variants J increases from 3 (very strong) instruments to 150 (weaker) instruments. However, this coincides with a reduction in the estimate’s standard error, so that its type I error rate rises sharply from 12% to 100%. MR-Egger regression returns approximately unbiased estimates for β for all values of J. As J increases the power of the MR-Egger test to detect directional pleiotropy increases from around 5% to 95%. The type I error rate of MR-Egger regression to detect a causal effect is well controlled for J ≤ 50 variants, but for over 100 variants some type I error inflation is apparent. In summary, MR-Egger regression works well with large numbers of genetic variants (in the sense that it has an increased power to detect pleiotropy), as long as the variants are not too weak. Kotva anthem download 1095 Kotva anthem download 1095 Kotva anthem download 1095 Conclusion. Edit Locked. Kotva anthem download 1095 Kotva anthem download 1095

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